Selective gating of neuronal activity by intrinsic properties in distinct motor rhythms

This research has been supported by the Royal Society, Wellcome Trust (089319), and the Biotechnology and Biological Sciences Research Council (BB/L0011X/1). I thank Drs. Steve Soffe, Alan Roberts, Erik Svensson, Hong-Yan Zhang, and Stefan Pulver for commenting on the manuscript.Many neural circuits show fast reconfiguration following altered sensory or modulatory inputs to generate stereotyped outputs. In the motor circuit of Xenopus tadpoles, I study how certain voltage-dependent ionic currents affect firing thresholds and contribute to circuit reconfiguration to generate two distinct motor patterns, swimming and struggling. Firing thresholds of excitatory interneurons [i.e., descending interneurons (dINs)] in the swimming central pattern generator are raised by depolarization due to the inactivation of Na+ currents. In contrast, the thresholds of other types of neurons active in swimming or struggling are raised by hyperpolarization from the activation of fast transient K+ currents. The firing thresholds are then compared with the excitatory synaptic drives, which are revealed by blocking action potentials intracellularly using QX314 during swimming and struggling. During swimming, transient K+ currents lower neuronal excitability and gate out neurons with weak excitation, whereas their inactivation by strong excitation in other neurons increases excitability and enables fast synaptic potentials to drive reliable firing. During struggling, continuous sensory inputs lead to high levels of network excitation. This allows the inactivation of Na+ currents and suppression of dIN activity while inactivating transient K+ currents, recruiting neurons that are not active in swimming. Therefore, differential expression of these currents between neuron types can explain why synaptic strength does not predict firing reliability/intensity during swimming and struggling. These data show that intrinsic properties can override fast synaptic potentials, mediate circuit reconfiguration, and contribute to motor–pattern switching.Publisher PDFPeer reviewe

Relationship between the symmetry energy and the single-nucleon potential in isospin-asymmetric nucleonic matter

In this contribution, we review the most important physics presented originally in our recent publications. Some new analyses, insights and perspectives are also provided. We showed recently that the symmetry energy $E_{sym}(\rho)$ and its density slope $L(\rho)$ at an arbitrary density $\rho$ can be expressed analytically in terms of the magnitude and momentum dependence of the single-nucleon potentials using the Hugenholtz-Van Hove (HVH) theorem. These relationships provide new insights about the fundamental physics governing the density dependence of nuclear symmetry energy. Using the isospin and momentum (k) dependent MDI interaction as an example, the contribution of different terms in the single-nucleon potential to the $E_{sym}(\rho)$ and $L(\rho)$ are analyzed in detail at different densities. It is shown that the behavior of $E_{sym}(\rho)$ is mainly determined by the first-order symmetry potential $U_{sym,1}(\rho,k)$ of the single-nucleon potential. The density slope $L(\rho)$ depends not only on the first-order symmetry potential $U_{sym,1}(\rho,k)$ but also the second-order one $U_{sym,2}(\rho,k)$. Both the $U_{sym,1}(\rho,k)$ and $U_{sym,2}(\rho,k)$ at normal density $\rho_0$ are constrained by the isospin and momentum dependent nucleon optical potential extracted from the available nucleon-nucleus scattering data. The $U_{sym,2}(\rho,k)$ especially at high density and momentum affects significantly the $L(\rho)$, but it is theoretically poorly understood and currently there is almost no experimental constraints known.Comment: 9 pages, 6 figures, Review paper, Contribution to the "Topical Issue" on "Nuclear Symmetry Energy" in European Physical Journal

Making in situ whole-cell patch-clamp recordings from Xenopus laevis tadpole neurons

Xenopus laevis tadpoles have been an excellent, simple vertebrate model for studying the basic organization and physiology of the spinal cord and motor centers in the brainstem. In the past, intracellular recordings from the spinal and brainstem neurons were primarily made using sharp electrodes, although whole-cell patch-clamp technology has been around since the early 1980s. In this protocol, I describe the dissections and procedures needed for in situ whole-cell patch-clamp recording, which has become routine in tadpole neurophysiology since the early 2000s. The critical step in the dissections is to delicately remove some ependymal cells lining the tadpole neurocoele in order to expose clean neuronal somata without severing axon tracts. Whole-cell recordings can then be made from the somata in either current- or voltage-clamp mode.PostprintPeer reviewe

On the Mass-Period Distributions and Correlations of Extrasolar Planets

In addition to fitting the data of 233 extra-solar planets with power laws, we construct a correlated mass-period distribution function of extrasolar planets, as the first time in this field. The algorithm to generate a pair of positively correlated beta-distributed random variables is introduced and used for the construction of correlated distribution functions. We investigate the mass-period correlations of extrasolar planets both in the linear and logarithm spaces, determine the confidence intervals of the correlation coefficients, and confirm that there is a positive mass-period correlation for the extrasolar planets. In addition to the paucity of massive close-in planets, which makes the main contribution on this correlation, there are other fine structures for the data in the mass-period plane.Comment: to be published in AJ, tentatively in December 200